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KMID : 1101320110430030120
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Korean Journal of Clinical Laboratory Science
2011 Volume.43 No. 3 p.120 ~ p.123
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Waterborne Pathogens Identification in Public Bathroom by PCR-Reverse Blot Hybridization Assay
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Choi Seung-Gu
Song Woon-Heung
Lee Jae-Sang
Yang Byoung-Seon
Choi Myeong-Sik
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Abstract
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A total of 30 water samples were collected from 30 different public baths in Seoul ,Korea. Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to increase background rates of disease. Pathogens in water was filtered by nitrocellulose membrane with 0.45 ?m pore size. The membrane filters were analyzed by both cultivation and polymerase chain reaction (PCR) amplification of partial 16S rRNA gene. Various microorganisms including 4 Escherichia coli/Shigella spp. 1 Salmonella spp. 3 Pseudomonas aeruginosa and 2 Mycobacterium spp. were identified by reverse blot hybridization assay (REBA). PCR-REBA was able to identify many bacterial genera in one assay. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.
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KEYWORD
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Public bathroom, Wateborne pathogene, REBA
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